Research Group of
Nano-bio Engineering Research
Introduction
Department of Bio-Nano Science and Engineering, also called as Bio-Nano Engineering Laboratory, was built in 2005. It is composed of chemistry materials lab., nanoscale diagnosis technology lab., biochemistry and molecular biology lab. and cell culture lab., also shares with the art-state National Key Lab. of Micro/Nano Fabrication Technology, and Key Lab. for Thin Film and Microfabrication of Ministry of Education.
Its main aim is to explore the interdisciplinary area between life science and nanomaterial science, that is, bionanomaterials science. Bionanomaterials science has emerged as a new exciting field in which theoretical and experimental studies of structure and function of bionanomaterials has become a focus, and the importance of DNA, RNA, and peptides as bionanomaterials to the fundamental development in biology and nanomaterials has begun to be recognized. In particular, biochemistry, biophysics, biomechanics, thermodynamics, and electronic properties of DNA, RNA, and peptides, as well as intelligent composite biological materials, have become a new interdisciplinary frontier in life science and material science. DNA, RNA, and protein are nanosclae components important for the execution of the functions of life and are the best natural bionanomaterials. Interaction among these components weaves a complicated, optimized, yet perfect biological world. Exploration of nanoscale biological materials not only deals with structure and function of genes and proteins (including their recognition, binding, phase change, release of specific factors, induction of bioelectronic and biochemical signals, harmonious movement of cytoskeleton, and mechanical and thermal properties), but also involves the development of high-tech tools to analyze biological systems. There is an increasing need for a more systematic study of the basic issues involved in bionanomaterials and a more active participation of researchers in the application domain of such novel materials. Great advances have been and are being made in nanobiochip materials, nanoscale biomimetic materials, nanomotors, nanocomposite materials, interface biomaterials, and nanobiosensor and nano drug delivery systems, with enormous prospect in industrial, defense, and clinical medicine applications. We not only explore the interdisciplinary area of life science and nanomaterial science, develop novel theory, but also we fully use Nanomaterials and Bioengineering technologies to develop new generation of nanoscale diagnosis technology, and explore their real applications in clinical medicine.
Research and Development Direction
» Nanoscale Diagnosis Technology
To study and develop new detection technologies such as nanochips, nanoscale biosensors and molecular imaging based on these nanomaterials such as carbon nanotubes, quantum dots, dendrimer, gold nanoparticles, gold nanorods, magnetic nanoparticles and silver nanoparticles.
To further perfect biochip-based gastric cancer pre-warning system, and apply to clinical diagnosis.
» Nanoscale Therapeutic Technology
To develop nanoscale gene/drug delivery system, and integrate nanoscale biodegradable materials into traditional drugs, develop new generation of nano-drug.
To fully employ the absorbance properties of near infrared areas of nanoscale materials, develop new generation of tumor therapeutic technology.
» Nanomaterials synthesis and their biological effects and biosafety
To synthesize nanomaterials with specific function, and investigate their effects on human cells, environment, and safety, and investigate their application in biomedical engineering.
» Studies of gene structure and function
In our previous work, we found some genes associated with tumors, such as brcaa1, gcys-1 to gcys-18, etc., we continue to investigate their structure and function, and prepare their antibodies, and investigate their applications in tumor diagnosis and therapy.
Group Members
Prof. & Director : Daxiang Cui
Daxiang Cui, Chinese, studied medicine at Shanghai Second Military Medical University of China, receiving his B. S. degree in 1990. He studied at Department of Orthopedics of Tangdu hospital, receiving his M.S. degree in 1995, then he studied at department of biochemistry and molecular biology in Fourth Military Medical University, receiving his ph. D. degree in 1998. He was promoted to associated professor in 2000. He joined Max Planck Institute for Metals Research as visiting scientist in 2001 and was promoted to scientific staff in 2002. He became a professor in Institute of Micro/ Nano Science and Technology, Shanghai JiaoTong university in 2004. He became a gust professor in Consolidated Research Institute for Advanced Science and Medical Care of Waseda university in 2007. He has obtained one of Military Science-Tech Advance Second Award in 2001, one of Shanxi province Science-Tech Second Award in 2002, one of Shannxi Province Science-Tech Second Award in 2005, and one of Tech-Learn Achievement Second Award of China in 2002. He also obtain some patents, published 11 books as co-author, one book as chief-editor. He was also involved in development of the diagnosis chip and matched reagents, which obtained permission certifications from China (National Diagnosis reagent I type New Drug S20020025, and S 20050056). He also obtained the support from Shanghai Pujian Plan in 2006, he has taken on and finished several key projects, so far he takes on one key project of 863, one of sub-project of 973, one of specific nano-project.
His research interests mainly focus on nanoscale diagnosis and therapeutic technologies such as biochip and biosensor, biological effects and bio-safety of nanomaterials, and structure and function of gene and protein.
Associate Prof. : Feng Gao
Feng Gao, Chinese, studied at Department of Chemistry in East China Normal University of China, receiving her B. S. degree in 1982. She studied at Department of Radioimmunoassay in Shanghai Institute of Nuclear Research, Academia Sinica., receiving her M.S. degree in 1987. She worked in Shanghai Institute of Nuclear Research, Academia Sinica., from 1990 to 2002. She was promoted to associated professor in 1998. Now she worked as an associate professor in Institute of Micro/ Nano Science and Technology, Shanghai JiaoTong University. She obtained the support from Shanghai Development Foundation of Science and Technology (No. 03ZR14057) and National Natural Science Fund of China (No.30471599). Her research interests mainly focus on investigation of immunoassay based on nanoparticles labelled biomolecule and preparation of nanoparticles, such as magnetite nanoparticles, quantum dot, gold nanoparticles, platinum nanoparticles, and silver nanoparticles, Applications of magnetic microspheres in cell separation, protein purification and DNA/RNA separation, Self-assembly of nano-materials by bio-molecules.
Associate Prof. : Rong He
Rong He:Chinese. She got her Master degree of polymer materials and engineering at Northwest Polytechnical University. She studied at Department of chemistry and chemical engineering in Shanghai JiaoTong University and received her ph. D. degree in 2003. She worked as a Lecturer in Department of chemistry and chemical engineering in Wuxi University of Light Industry from 1995 to 2000. Now she worked as Associate Professor in Department of Bio-Nano Science and Technology in Shanghai Jiaotong University. Published over 30 articles and applied 6 patents. She also obtained the support from NSF.
Her research interests mainly focus on Biological Activity and Bio-safety of Nano-materials, Fabrication, purification and medical application of bio-nanomaterials such as CNT, Quantum dot, PAMAM molecules and Interaction mechanism between bio-nanomaterials and human normal or tumor cells.
International Cooperation
Prof. Beate Brand-Saberi
Prof. Cengiz S. Ozkan
Dr. Hong Zhang
National Cooperation
Prof. Jian Ni
Prof. Rina Wu
Associated Prof. Yixin Zhang
Assistant professor Mao Yang
Main publications
- Bifeng Pan, Daxiang Cui, Yuan Sheng, Cengiz S. Ozkan, Feng Gao, Rong He, Qing Li, Ping Xu, Tuo Huang. Dendrimer-modified magnetic nanoparticles enhance efficiency of gene delivery system. Cancer Research, 2007;67(17):8156-8163.
- Daxiang Cui, Furong Tian, Sean R. Coyer, Jicun Wang, Befeng Pan, Feng Gao, Rong He and Yafei Zhang. Effects of Antisense-myc-conjugated Single-Walled Carbon Nanotubes on HL-60 cells. J. Nanoscience & Nanotechnology, 2007;7:1639-1641.
- Daxiang Cui. Advance and prospects of biomolecules functionalized carbon nanotubes. J. Nanoscience & Nanotechnology, 2007; 7:1298-1314.
- Rong He, Xioagang You, Jun Shao, Feng Gao, Bifeng Pan and Daxiang Cui. Core/shell fluorescent magnetic silica-coated composite nanoparticles for bioconjugation. Nanotechnology 2007, 18: 315601.
- Xiaogang You, Rong He, Feng Gao, Jun Shao, Bifeng Pan, and Daxiang Cui. Hydrophilic high-luminescent magnetic nanocomposites. Nanotechnology, 2007; 18: 035701.
- Bifeng Pan, Daxiang Cui, Cengiz Ozkan, Ping Xu, Tuo Huang, Qing Li,, Hao Chen, Fengtao Liu, Feng Gao, Rong He.DNA-Templated Ordered Array of Gold Nanorods in One and Two Dimensions.Journal of Physical Chemistry C, 2007;111(34):12572-12576.
- Hao Chen, Feng Gao, Rong He,Daxiang Cui. Chemiluminescence of luminol catalyzed by silver nanoparticles. Journal of Colloid and Interface Science, 2007; 315: 158-163.
- Bifeng Pan, Daxiang Cui, Rong He, Feng Gao, Yafei Zhang. Covalent attachment of quantum dot on carbon nanotubes. Chemical Physics Letters, 2006;417:419–424.
- Bifeng Pan, Daxiang Cui, Feng Gao, Rong He. Growth of multi-amine terminated poly(amidoamine)dendrimers on the surface of carbon nanotubes. Nanotechnology, 2006; 17:2483-2489.
- Limei Ao, Feng Gao, Bifeng Pan, Rong He, Daxiang Cui.Fluoroimmunoassay for Antigen Based on Fluorescence Quenching Signal of Gold Nanoparticles. Analytical. Chemistry, 2006;78:1104-1106.
- Daxiang Cui, LiZhang, XiaojunYan, Lingxia Zhang, Junrong Xu, Yanhai Guo, Guiqiu Jin, Giovani Gomez, Ding Li, Jinrong Zhao, Fenchan Han, Ju Zhang, Jiale Hu, Daiming Fan, Huajian Gao. A microarray-based gastric cancer prewarning system. World J. Gastroentrol., 2005;11(9):1273-1282.
- Bifeng Pan, Limei Ao, FengGao, Hongye Tian, Rong He, Daxiang Cui. End-end self-assembly and Colorimetric Characterization of Gold Nanorod and Nanosphere via Oligonucleotide hybridization. Nanotechnology, 2005;16:1776-766.
- Yuedong Han,Yi Huan,Jinlan Deng,Feng Gao,Bifeng Pan,Daxiang Cui.Expression of Single-Chain Fv Gene Specific for gama-Seminoprotein by RTS and Its Biological Activity Identification. Biotechnology Progress,2006; 22(4):1084-1089.
- Daxiang Cui, Furong Tian, Cengis Ozkan, Mao Wang, Huajian Gao. Effect of single wall carbon nanotubes on human HEK293 cells. Toxicology Letter, 2005; 155(1): 77-83.
Books
Nanotoxicity, American Science Press, 2006.
Nanotechnology-Theory and Modeling. SPIE press, 2006.
Medicinal Chemistry and Pharmacological Potential of Fullerenes and Carbon Nanotubes,Springer press, 2007.
Polymer fuel cells membrane technology, co-chief editor, Beijing High-Education press, 2007.